Fig 14. Schematic representation of the experimental design.

Multiparous timed pregnant olive baboons (n = 4) were infected subcutaneously (1x10⁴ ffu, 1 ml volume, strain H/FP/2103) on Day 0. Maternal blood, urine, CSF and saliva were obtained on the indicated days for each animal. The gestational age at the time of infection ranged from 97–107 days gestation (dG). One dam was euthanized at day 7 post infection (Dam 1), two dams on day 14 post infection (Dams 2,3) and on at 21 days post infection for collection of maternal and fetal tissues. The gestational age range at necropsy was 109–121 dG; a timed pregnant control dam was euthanized at 116 dG.