Skip to main content
. 2018 Jun 29;33(1):114–125. doi: 10.1096/fj.201800019RR

Figure 4.

Figure 4

Debris-stimulated tumor growth is OPN dependent. A) Debris-stimulated MC38 (1 × 104 living MC38 coinjected with 9 × 105 5-FU–generated MC38 tumor cell debris) tumor growth in OPN KO mice compared with WT mice (n = 4–5 mice/group). B) ELISA quantification of OPN in plasma from WT and OPN KO mice that were injected with debris-stimulated MC38 tumors or control non-tumor–bearing WT and OPN KO mice (n = 4–5/group). C) Debris-stimulated CT26 tumor growth (1 × 104 living CT26 coinjected with 9 × 105 CT26 tumor cell debris) treated with anti-OPN Ab or control IgG (20 µg Ab/mouse every 3 d; n = 5 mice/group). D) ELISA quantification of OPN in plasma from mice that were injected with debris-stimulated CT26 tumors treated with control IgG or anti-OPN Ab (n = 5 mice/group). E) CT26 tumors treated with 5-FU (30 mg/kg every 3 d) and/or anti-OPN Ab (20 µg/mouse every 3 d; n = 5 mice/group). Data are presented as means ± sem. Two-tailed Student’s t test for final tumor measurements were used throughout unless specified; 1-way ANOVA analysis was used for comparison of 3 or more groups. **P < 0.01, ***P < 0.001.