Figure 5.

Ccsp-GR^−/− mice retain sensitivity to anti-inflammatory effects of Dex during LPS challenge. GR^WT and Ccsp-GR^−/− mice (median age, 12 wk) were exposed to nebulized LPS at CT0 and culled 5 h later. The intraperitoneal injection of either Dex (1 mg/kg) or saline (vehicle) took place 1 h before LPS exposure. A, B) Quantification (A) of total cells (left) and neutrophils (right) in BAL fluid. Expression (B) of Cxcl5, Cxcl1, and Il6 in lung homogenate after challenge. n = 6–10, and data were analyzed with 2-way ANOVA, followed by Sidak’s multiple comparisons test for effects within genotype. C) Hierarchical clustering of proinflammatory cytokines/chemokines from Nanostring analysis (see Supplemental Fig. 6 for full heat map of Dex-responsive genes, and Supplemental Table 1 for list of all genes analyzed). Data represent means ± se. *P < 0.05, **P < 0.01, ***P < 0.001.