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. 2018 Jun 29;33(1):195–203. doi: 10.1096/fj.201800509R

Figure 3.

Figure 3

TRPV4 knockdown/deletion increases phosphorylation (Y1175) and membrane translocation of VEGFR2. A) Immunofluorescence images of p-VEGFR2 (red) in control siECs and TRPV4 siECs. Arrows denote membrane localization of p-VEGFR2. Nuclei were stained with DAPI (blue). The graph depicts the quantitative analysis of percent cells with membrane localization of p-VEGFR2 from control and TRPV4 siECs. Data presented are means ± sem of 3 independent experiments. B) Western blot analysis represents the expression levels of total and p-VEGFR2 in whole cell lysates from control siEC and TRPV4 siEC. Relative p-VEGFR2 expression was quantified by normalizing with total VEGFR2. The graph represents the quantitative analysis of relative p-VEGFR2 expression from the whole cell lysates of control and TRPV4 siEC. C) Western blots demonstrating the expression levels of total and p-VEGFR2 in membrane fractions from control and TRPV4 siEC. β1-integrin expression was used as a loading control for plasma membrane fractions. The graph depicts the quantitative analysis of relative p-VEGFR2 expression from the membrane fractions of control and TRPV4 siEC. The given results are means ± sem from 3 independent experiments. *P ≤ 0.05. Scale bar, 10 μm.