Figure 4.

TRPV4 knockdown increases YAP nuclear translocation in HMECs-1 via the rho kinase pathway. A) Immunofluorescence images of YAP (green) in control siECs and TRPV4 siECs in the presence or absence of Y-27632 (Y27; 10 μM). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. B) The graph represents the quantitative analysis of the percentage of cells with nuclear localization of YAP from control siEC, TRPV4 siEC, control siEC + Y27, and TRPV4 siEC + Y27. Data presented are means ± sem of 3 independent experiments. *P ≤ 0.05.