Figure 3.

Differential expression (DE) analysis. A) PCA of 18 MSC-derived pellet samples. Samples were closely related by days, and there was minor donor-to-donor variation. B) PCA of MSC-derived pellet samples vs. human cartilage at different developmental stages. C) Expression patterns of MSC markers and markers of potential lineages. Gene expression was represented as log₂ fold change compared to d 0 samples. D) K-means clustering grouped DE genes into 4 patterns. Gene expression was represented as log₂ fold change compared to d 0 samples. E) Bar graphs of enriched pathways from gene ontology analysis. Datasets for human cartilage were retrieved using GEO accession number GSE106292 (79).