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Dietary epicatechin reversed age-related metabolomic changes associated with NAD pathway in aged mice. Principal component analysis revealed perfect separation between YC and OC, and EC intake significantly shifted back to YC (A). Random forest analysis resulted in a predicted accuracy of 78% between groups (B), and levels of 6 metabolites were significantly changed by EC compared with the OC group (C–H). #P < 0.05 EC vs. YC; *P < 0.05 EC vs. OC (n = 12/group).
