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AICAR activates Akt and downstream protein translation regulators. Immunoblotting in total heart lysate made from left ventricle sections. Densitometry analysis was performed normalizing phosphoprotein to total target protein levels: peEF2/total eEF2 (A), p-Akt/total Akt and p-Erk1/2/total Erk1/2 (B), and p-4EBP1/total 4EBP1 and p-S6/total S6 (C). Total protein staining for all gels can be found in the Supplemental Data.
