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. 2018 Aug 13;33(1):1199–1208. doi: 10.1096/fj.201800841R

Figure 5.

Figure 5

Matrix stiffness regulates endothelial integrity in vivo. A) Confocal microscopy images showing tumor sections of MMTV-PyMT mice treated with or without (Ctrl) BAPN. Tumor sections were stained for p-Src and VE-cadherin (VE-Cad). B) Corresponding quantification of p-Src signal around endothelial adherens junctions showing matrix stiffening increased amount of p-Src around adherens junctions (n = 3; 10–15 imaging fields included in each condition for each experiment). C) Confocal microscopy images showing p-FAK expression of tumor sections derived from MMTV-PyMT mice with or without BAPN treatment. D) Corresponding quantification of p-FAK expression of tumor endothelial cells showing endothelial cells of stiffer tumors maintain higher FAK activation. E) Confocal microscopy images showing tumor sections of MMTV-PyMT mice treated with or without BAPN. Tumor sections were stained for VE-cadherin and phosphorylated VE-cadherin (n = 3; 10–15 imaging fields included in each condition during each experiment). Data are presented as means ± se. ****P < 0.0001.