Mouse Mks6 mutant alleles. A) Allele schematics drawn to scale: WT (Mks6WT), congenital gene trap allele with β-galactosidase cassette indicated (β-geo) in intron 11 (Mks6GT), conditional allele with LoxP sites indicated (P) flanking exons 6 and 7 (Mks6FL), and conditional mutant allele (Mks6Δ). Genotyping primers for the conditional and conditional mutant alleles are indicated (F, R1, and R2 arrows). B) qRT-PCR indicates loss of transcript in Mks6GT MEFs. Fold change was calculated as 2−ΔΔCt.
C) Mks6GT embryos presented with variable phenotypes at different embryonic stages. *P < 0.05 (Student’s t test).