Figure 3.

Variable cystic kidney phenotype in Mks6^Δ mutants. A) Longitudinal MRI, terminal µCT, and histology studies of Mks6^Δ mutant kidneys. Mks6^Δ mice (9982, 9985, 9986 littermates) show variability in cyst progression, kidney size, and cyst growth over time after Cre induction on P7 compared with control littermates (9984). Terminal µCT and histology [hematoxylin and eosin (H&E)] analysis was performed on kidneys at P63. Original scale bars, 1 mm. B) PCR genotyping for Mks6^FL (Flox), Mks6^Δ (Δ), and Cre alleles was performed to evaluate potential correlations between cyst severity and the extent of Cre-mediated deletion. C) Kidney function and phenotypic severity were also assessed by measuring TKV, TKV/BW, serum creatinine levels (mg/dl), and BWs post-Cre induction (Weeks PI).