Figure 5.

Retinal degeneration phenotype in Mks6^Δ mutants. A, B) Hematoxylin and eosin staining of retina sections from Mks6^FL and Mks6^Δ mutants from juveniles (P21) and adults (20 wk). INL, inner nuclear retinal layer; ONL, outer nuclear retinal layer; RPE, retinal pigmented epithelia retina layer. Original scale bars, 50 µm. C) Morphometric analysis of nuclei counts at different distances from the optic nerve head using Student’s t test reveals only slight retinal degeneration in juveniles (P21) compared with statistically significant retinal degeneration in adults (20 wk). D) TUNEL staining images for apoptosis (red) in P21 retinas. DAPI-stained nuclei are blue. Original scale bar, 50 µm. E) Graph of TUNEL quantification in P21 retinas. Kruskal-Wallis nonparametric ANOVA with post hoc Mann-Whitney comparisons yielded P < 0.05; means ± sem, n = 6 per group.