Figure 8.

Analysis of the olfactory system in Mks6^Δ mutants. A, B) Global coronal section of the nasal cavity with the OE and RE from Mks6^FL (Control) and mutant (Mks6^Δ). Immunofluorescence for a marker for mature OMPs, and cilia markers (acetylated α-tubulin and ACIII). Closer examination of the OE and RE border (demarcated with dashed line) from Mks6^FL and Mks6^Δ mutant mice demonstrates the presence of olfactory sensory cilia and motile respiratory cilia in both mice. C, D) Coronal section of the OB medial surface from Mks6^FL (Control) and mutant (Mks6^Δ). DAPI stained with individual glomeruli (red outlines) and TH immunolabeling demonstrated no difference in glomerular size and trans-synaptic activation, respectively. Original scale bars, 500 µm (A, B, upper), 50 µm (A, B, lower); 100 µm (C, D), n = 3 per group.