A comparison of the amino acid sequences of the 3 TIMPs from the sea lamprey (P. marinus) and the 2 from inshore hagfish (E. burger) with the 4 human (Homo sapiens) and elephant shark (Callorhinchus milii) TIMPs. The sequences were aligned by using T-coffee (https://www.ebi.ac.uk/Tools/msa/tcoffee/) and shaded by using Boxshade (embnet.vital-it.ch/software/BOX_form.html). Residues with black backgrounds are identical in ≥7 sequences. CALM, Callorhinchus milii; HAGF, Eptatretus burger; HU, Homo sapiens; LAMP, Petromyzon marinus; TIMPa, Ta; TIMPb, Tb; TIMPc; TcTIMP1, T1; TIMP2, T2; TIMP3, T3.