Table 3.

Proportions (mean ± SD)^a of black flounder eggs fertilized after 1 min treatment with 0.003% trypsin in Ringer's.

	Sperm concentration at insemination
Egg treatment with trypsin	2.7 × 107/ml	2.7 × 106/ml	0.4 × 106/ml
No (control)	87.7 ± 3.8	75.7 ± 6.3	58.2 ± 6.7
Yes	46.3 ± 5.1*	34.7 ± 5.9*	9.3 ± 4.5*

^aMean ± SD of three experiments using a single male and a single female. About 100 eggs were used for each determination. Fertilization rates for all trypsin treated eggs were significantly different (P < 0.05; asterisk) from the untreated eggs, regardless of the sperm concentration used.