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Fertilization of black flounder, goldfish, and loach eggs in normal and Ca2+-free media.
| Total no. of eggs | ||||
|---|---|---|---|---|
| Species | Insemination medium | No. of exp. | Inseminated | Fertilized (%) |
| Black flounder | ASW | 3 | 396 | 360 (91) |
| Ca2+-free ASW | 3 | 404 | 0 (0) | |
| Goldfish | 1/100 Ringer's | 2 | 135 | 128 (95) |
| Ca2+-free 1/100 Ringer's | 2 | 127 | 0 (0) | |
| Loach | 1/100 Ringer's | 2 | 87 | 77 (89) |
| Ca2+-free 1/100 Ringer's | 2 | 66 | 0 (0) | |
