. 2017 Mar 24;96(4):780–799. doi: 10.1093/biolre/iox015

Table 7.

Parameters used for estimation of the presence or absence of micropylar sperm attractant (MISA) in fish egg.

Species (Micropyle type)	Staining (+) or no staining (–) of micropylar region by treatment		Loss of staining ability of micropylar region by trypsin	Efficiency of sperm entry in micropyle is affected (+) or not affected (–) by extracellular Ca²⁺	Presence (+) or absence (–) of MISA
Species (Micropyle type)	CB	FITC-WGA	Loss of staining ability of micropylar region by trypsin		Presence (+) or absence (–) of MISA
Herring (I)	+	+	Yes	+	+
Flounder (I)	+	+	Yes	+	+
Trout (II)	+	+	Yes	+	+
Anchovy (II)^a	–	+	Yes	+	+
Goldfish (III)	–	–^b	NA	–	–
Loach (III)	–	–^b	NA	–	–
Zebrafish (III)	–	–^b	NA	–	–

^aNote that anchovy micropyle is not stained by CB, but is stained by FITC-WGA.

^bOther FITC-conjugated lectins tested (ConA, UEA, RCA, and LCA) did not stain micropylar region of eggs of these fish.

NA: not applicable