Figure 4.

The expression of CXCR4 and CXCL12 in human granulosa cell cultures. Human granulosa cells isolated from follicle aspirates of IVF patients were cultured for 6 days and then treated without (Control) or with hCG (1 IU/ml) for indicated hours. The levels of mRNAs for CXCR4 (A) and CXCL12 (B) were measured by real-time PCR and normalized to the RNA18S5 in each sample. Experiments were repeated five times, each with different cell samples. Bars with no common superscripts are significantly different (P < 0.05).