Figure 5.

Regulation of PGR expression in human granulosa cells. Cultured human granulosa cells were treated with or without hCG (1 IU/ml) for 12 h. (A) Real-time PCR analyses were performed to measure the levels of PGR mRNA and their levels were normalized to the RNA18S5 in each sample. (B) Immunocytochemistry analysis showed strong positive staining for PGR protein (green/yellow) in the nucleus of granulosa cells stimulated with hCG. Propidium iodide (red) was used to stain the nucleus of cells. Experiments were repeated four times, each with different cell samples. Bars with no common superscripts are significantly different (P < 0.05).