Figure 6.

Effects of inhibitors on the level of mRNA for CXCR4 and PGR and progesterone production in human granulosa cells. Cultured human granulosa cells were treated with or without hCG (1 IU/ml) in the absence or presence of inhibitors or activator (RU486; 10 μM, AG1478; 5 μM and CoCl₂; 100 μM) and cultured for 12 or 24 h. Real-time PCR analyses were performed to measure the levels of mRNA for CXCR4 (A) and PGR (B), and their levels were normalized to the RNA18S5 in each sample. The concentration of progesterone (C) was measured in the conditioned media of human granulosa cell cultures. Experiments were repeated three to four times, each with different cell samples. Bars with no common superscripts in each time point are significantly different (P < 0.05).