Figure 7.

Effects of CXCL12 and AMD3100 in human granulosa cells. (A) Human granulosa cells were treated with CXCL12 (250 ng/ml) in the absence or presence of hCG (1 IU/ml) and cultured for 24 h. At the end of culture, the cells were subjected to MTS assay which measures cell viability. (B) Human granulosa cells were treated with CXCL12 (250 ng/ml) or AMD3100 (50 nM) in the absence or presence of hCG (1 IU/ml). Real-time PCR analyses were performed to measure the levels of CXCR4 (A and B) mRNAs, and their levels were normalized to the RNA18S5 in each sample. Experiments were repeated four times, each with different cell samples. Bars with no common superscripts are significantly different (P < 0.05).