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. Author manuscript; available in PMC: 2019 Jan 31.
Published in final edited form as: J Immunol Methods. 2018 Oct 10;463:137–147. doi: 10.1016/j.jim.2018.10.003

Figure 1 -. Experimental Setup:

Figure 1 -

An overview of sample processing. Donor-A gave ten 10ml draws of blood and Donor-B gave three. All blood draws were taken at the same time and each processed into a corresponding sample RNA pool. Aliquots of the RNA are used to create the amplified PCR product, aliquots of which are used to make multiple sequence libraries. Multiple libraries from the same sample RNA pool are compared to evaluate the coverage of the RNA-populations and multiple libraries from different sample RNA pools are compared to evaluate the Total-population.