Table 3. The refSNP and thermal conditions for HRM analysis.
| Polymorphism | refSNP | Primer sequence F-forward, R-reverse |
Thermal conditions | |
|---|---|---|---|---|
| XRCC1 | Arg399Gln | rs25487 | F 5′-TAAGGAGTGGGTGCTGGACT-3′ R 5′-ATTGCCCAGCACAGGATAAG-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 58° C Extension 30 s for 72° C HRM 75–90° C |
| hMSH2 | Gly322Asp | rs4987188 | F 5′-GTTTTCACTAATGAGCTTGC-3′ R 5′-AGTGGTATAATCATGTGGGT-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 55° C Extension 30 s for 72° C HRM 75–90° C |
| XPD | Lys751Gln | rs13181 | F 5′-CCTCTGTTCTCTGCAGGAGGA-3′ R 5′-CCTGCGATTAAAGGCTGTGGA-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 55° C Extension 30 s for 72° C HRM 75–90° C |
| XRCC2 | Arg188His | rs3218536 | F 5′-TGTAGTCACCCATCTCTCTGC-3′ R 5′-AGTTGCTGCCATGCCTTACA-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 56° C Extension 30 s for 72° C HRM 75–90° C |
| RAD51 | -4719A/T | rs2619679 | F 5′-AGATAAACCTGGCCAACGTG-3′ R 5′-CCGTGCAGGCCTTATATGAT-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 57° C Extension 30 s for 72° C HRM 75–90° C |
| RAD51 | -4601A/G | rs5030789 | F 5′-AGATAAACCTGGCCAACGTG-3′ R 5′-CCGTGCAGGCCTTATATGAT-3′ |
PCR cycling (40 cycles) Denaturation 30 s for 95° C Annealing 30 s for 58° C Extension 30 s for 72° C HRM 75–90° C |