Table 1. Microcolumn affinity tests.
| Peptide | Sequence | [M+H]+calc. | Supernatant [M+H]+ | Last wash | Elution [M+H]+ |
|---|---|---|---|---|---|
| CD160 (1–20) | INITSSASQEGTRLNLIAbuTV-NH2 | 2101.36 | 2101.02 | n.o. | n.o. |
| CD160 (13–32) | Ac-RLNLIAbuTVWHKKEEAEGFVV-NH2 | 2394.77 | 2394.37 | n.o. | 2394.32 |
| CD160 (25–44) | Ac-EEAEGFVVFLAbuKDRSGDAbuSP-NH2 | 2193.37 | 2193.21 | n.o. | 2193.19 |
| CD160 (37–56) | Ac-DRSGDAbuSPETSLKQLRLKRD-NH2 | 2327.56 | 2327.17 | n.o. | n.o. |
| CD160 (49–68) | Ac-KQLRLKRDPGIDGVGEISSQ-NH2 | 2237.52 | 2237.10 | n.o. | n.o. |
| CD160 (61–80) | Ac-GVGEISSQLMFTISQVTPLH-NH2 | 2185.50 | 2185.13 | n.o. | 2185.23 |
| CD160 (73–92) | Ac-ISQVTPLHSGTYQAbuAbuARSQK-NH2 | 2212.47 | 2210.11 | n.o. | 2210.19 |
| CD160 (85–104) | Ac-QAbuAbuARSQKSGIRLQGHFFSI-NH2 | 2271.58 | 2271.43 | n.o. | 2271.15 |
| CD160 (97–116) | Ac-LQGHFFSILFTETGNYTVTG-NH2 | 2273.50 | - | - | - |
| *CD160(97-110) | Ac-LQGHFFSILFTETG-NH2 | 1637.83 | 1637.65 | n.o. | 1637.65 |
| *CD160(103-116) | Ac-SILFTETGNYTVTG-NH2 | 1543.67 | 1565.60 [M+Na]+ | n.o. | n.o. |
| CD160(109-133) | Ac-TGNYTVTGLKQRQHLEFSHNEGTLS-NH2 | 2859.07 | 2859.23 | n.o. | n.o. |
n.o. – not observed; - – not results.