Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Hamster |
Mesocricetus auratus, Syrian golden |
Mahidol University, Thailand |
NA | |
| Parasite (liver fluke) |
Opisthorchis viverrini, adult development stage |
(Papatpremsiri et al., 2015; Laha et al., 2007; Sithithaworn et al., 1997; Pinlaor et al., 2013) |
NA | Infective stage metacercariae of O. viverrini obtained from naturally infected, cyprinid freshwater fish from Mukdahan and other Isaan provinces, Thailand, and used to infect hamsters. |
| Parasite (liver fluke) |
Opisthorchis viverrini, metacercariae and newly excysted juveniles |
(Papatpremsiri et al., 2015; Laha et al., 2007; Sithithaworn et al., 1997; Pinlaor et al., 2013; Papatpremsiri et al., 2016) |
NA | Infective stage metacercariae of O. viverrini obtained from naturally infected, cyprinid freshwater fish from Isaan provinces, Thailand. |
| Commercial kit |
GeneArt CRISPR Nuclease Vector Kit |
Invitrogen | A21174 | |
| Serum | Anti-Ov-GRN-1 rabbit serum |
(Smout et al., 2009) | NA | |
| Antibody | Alexa Fluor 594- labeled anti-ACTA2 |
Abcam (Capone et al., 2016; Burwinkel et al., 2018) |
ab202368 | |
| Commercial reagent |
Agencourt AMPure XP beads |
Beckman | A63880 | |
| Commercial reagent |
GeneRead Adaptors I Set A |
Qiagen | 180985 | |
| Commercial kit |
QIAseq 1-step Amplicon Library Kit |
Qiagen | 180412 | |
| Commercial kit | GeneRead Library Quant Kit |
Qiagen | 180612 | |
| Human cholangiocyte cell line, H69 |
Cholangiocyte cell line derived by SV 40 transformation |
(Smout et al., 2015; Grubman et al., 1994) |
H69; RRID: CVCL_8121 |
|
| Commercial kit |
Picro Sirius Red Stain Kit (Connective Tissue Stain) |
Abcam | ab150681 |