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. 2019 Jan 22;129(2):863–874. doi: 10.1172/JCI123980

Figure 8. Coexpression of PDE6C and R565Q with AIPL1 and Pγ in HEK293T cells.

Figure 8

(A) Confocal immunofluorescence images of HEK293T cells cotransfected with PDE6C or R565Q (red, anti-PDE6C) and the AIPL1-Pγ vector (AIPL1: blue, anti-HA; Pγ: green, EGFP fluorescence). (B) Immunoblot analysis of extracts of HEK293T cells cotransfected with PDE6C or R565Q and AIPL1 or the AIPL1-Pγ vector using anti-Flag (PDE6C), anti-EGFP (P), and anti-HA (AIPL1) antibodies. Lanes contain equal amounts of protein. From 4 similar experiments, the level of R565Q protein expression is reduced to 57% ± 9% (mean ± SEM) of that for PDE6C. (C) Scheme of fractionation of lysates from HEK293T cells cotransfected with PDE6C (or R565Q), AIPL1, and Pγ, and immunoblot analysis in the scheme fractions 1–5 with anti-Flag antibody. The findings suggest that the distributions of PDE6C and R565Q in HEK293T cells are qualitatively similar, but the amount of R565Q protein expression is reduced.