FIGURE 1.

GRPs differentiate into astrocytes in vitro. Undifferentiated AP+ GRPs maintained a glial progenitor-like morphology in culture (a). When stimulated to differentiate into astrocytes, AP+ GRPs acquired an astrocyte-like morphology (b). Prior to astrocyte differentiation, nearly all AP+ cells in the GRP culture expressed nestin (c) but did not express GFAP (e), while differentiated AP+ GRP-derived astrocytes did not express nestin (d) but highly expressed GFAP (f). Quantification of nestin and GFAP expression by AP+ cells (g). Human dermal fibroblasts in culture displayed a fibroblast-like morphology (h). Scale bar: 50 μm