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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Glia. 2018 Dec 11;67(3):452–466. doi: 10.1002/glia.23555

FIGURE 4.

FIGURE 4

GRPs did not affect lesion size in the cervical spinal cord or morphological innervation at the diaphragm neuromuscular junction. Using whole-mount immunohistochemistry on the ipsilateral hemidiaphragm, we labeled phrenic motor axons all the way to their presynaptic terminals with SMI-312 and SV-2 (a) and postsynaptic nicotinic acetylcholine receptors with Alexa-conjugated α-bungarotoxin (b). Nearly 100% of NMJs at all three muscle sub-regions were completely intact, with compete apposition of the pre-synaptic nerve terminal and the post-synaptic receptors (c). There were no differences in the percentage of innervated and denervated NMJs between fibroblasts and GRPs at any subregion (d). There was no difference between fibroblast and GRP animals in the rostral-to-caudal length of the lesion (e)