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. 2018 Sep 27;104(2):347–359. doi: 10.3324/haematol.2018.195131

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Figure 4. — Ectopic expression of miR-497 induces cell cycle arrest and reduces growth in vivo of NPM-anaplastic lymphoma kinase (ALK)-positive(⁺) lymphoma cells. NPM-ALK⁺ ALCL COST, KARPAS-299 (KARPAS) and SU-DHL1 cells were transfected with negative control miRNA (miR-CTL) or mimic miR-497 (miR-497). Cell cycle was analyzed (A) and caspase 3/7 activity was measured (B). Data were normalized against the miR-CTL condition. Each experiment was repeated 3 times. (C) NPM-ALK⁺ COST, KARPAS-299 (KARPAS) and SU-DHL1 cells transfected either with miR-CTL or miR-497 were injected subcutaneously in the left or right flank of immunodeficient mice, respectively (n=6). Tumor volume was evaluated over time by caliper measurements. Data represent mean±Standard Error of Mean, *P<0.05; **P<0.001; ***P<0.0001; ns: not significant; unpaired two-tailed Student’s t-test with Welch’s correction. (D) Micrographs of hematoxylin & eosin staining of excised miR-CTL or miR-497 tumors (original magnification ×40). Arrows indicate cells with phenotypical hallmarks of cellular degeneration.