TABLE 2.
Numbers of nontuberculous mycobacteria isolated and percent sensitivities of different culture test methods for recovery of nontuberculous mycobacteria
| Organism | Total no. of mycobacterial isolatesb | No. of mycobacterial isolates (% sensitivity)a |
|||||
|---|---|---|---|---|---|---|---|
| RGM30 | RGM35 | MGIT | 7H11 | AFC | MGIT+RGM30 | ||
| Rapidly growing mycobacteria | |||||||
| M. abscessus complex | 28 | 27 (96.4) | 27 (96.4) | 25 (89.3) | 24 (85.7) | 25 (89.3) | 28 (100) |
| M. chelonae | 5 | 5 (100) | 0 | 0 | 0 | 0 | 5 (100) |
| M. fortuitum group | 6 | 4 (66.7) | 3 (50) | 2 (33.3) | 2 (33.3) | 3 (50) | 4 (66.7) |
| M. immunogenum | 1 | 1 (100) | 0 | 0 | 0 | 0 | 1 (100) |
| M. llatzerense | 1 | 1 (100) | 0 | 0 | 0 | 0 | 1 (100) |
| M. mucogenicum group | 16 | 16 (100) | 1 (6.3) | 0 | 1 (6.3) | 1 (6.3) | 16 (100) |
| Total | 57 | 54 (94.7) | 31 (54.4) | 27 (47.4) | 27 (47.4) | 29 (50.9) | 55 (96.5) |
| Slowly growing mycobacteria | |||||||
| M. avium complex | 48 | 29 (60.4) | 32 (66.7) | 42 (87.5) | 34 (70.8) | 45 (93.8) | 45 (93.8) |
| M. avium | 24 | 13 (54.2) | 17 (70.8) | 21 (87.5) | 16 (66.7) | 23 (95.8) | 22 (91.7) |
| M. colombiense | 1 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) |
| M. intracellulare/M. chimaera | 22 | 15 (68.2) | 14 (63.6) | 19 (86.4) | 16 (72.7) | 20 (90.9) | 21 (95.5) |
| M. yongonense | 1 | 0 | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) |
| M. gordonae | 14 | 10 (71.4) | 0 | 5 (35.7) | 0 | 5 (35.7) | 14 (100) |
| M. lentiflavum | 1 | 0 | 1 (100) | 1 (100) | 0 | 1 (100) | 1 (100) |
| M. paragordonae | 7 | 4 (57.1) | 0 | 3 (42.9) | 0 | 3 (42.9) | 7 (100) |
| M. paraffinicum | 1 | 0 | 0 | 0 | 1 (100) | 1 (100) | 0 |
| M. simiae | 1 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) |
| Mycobacterium sp. | 4 | 4 (100) | 1 (25) | 0 | 0 | 0 | 4 (100) |
| Total | 76 | 48 (63.2) | 35 (46.1) | 52 (68.4) | 36 (47.4) | 56 (73.7) | 72 (94.7) |
| Grand total | 133 | 102 (76.7) | 66 (49.6) | 79 (59.4) | 63 (47.4) | 85 (63.9) | 127 (95.5) |
a
MGIT, mycobacterial growth indicator tube; 7H11, Middlebrook 7H11 agar. RGM30 and RGM35 are RGM media incubated at 30°C and 35°C, respectively. AFC is a culture method using the MGIT system and Middlebrook 7H11 agar.
b
That is, the total number of nontuberculous mycobacteria isolated from a combination of all culture test methods (RGM30, RGM35, the MGIT system, and Middlebrook 7H11 agar).