TABLE 3.
Cumulative number and percent recovery of nontuberculous mycobacteria isolated from RGM media incubated at 30°C (RGM30) and 35°C (RGM35)
| Organism | Total no. of mycobacterial isolatesa | No. of mycobacterial isolates (% recovery) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RGM30 |
RGM35 |
||||||||||||
| D4 | D7 | D10 | D14 | D21 | D28 | D4 | D7 | D10 | D14 | D21 | D28 | ||
| Rapidly growing mycobacteria | |||||||||||||
| M. abscessus complex | 28 | 26 (92.9) | 27 (96.4) | 27 (96.4) | 27 (96.4) | 27 (96.4) | 27 (96.4) | 26 (92.9) | 27 (96.4) | 27 (96.4) | 27 (96.4) | 27 (96.4) | 27 (96.4) |
| M. chelonae | 5 | 3 (60) | 3 (60) | 4 (80) | 5 (100) | 5 (100) | 5 (100) | 0 | 0 | 0 | 0 | 0 | 0 |
| M. fortuitum group | 6 | 1 (16.7) | 3 (50) | 3 (50) | 3 (50) | 4 (66.7) | 4 (66.7) | 0 | 1 (16.7) | 1 (16.7) | 1 (16.7) | 3 (50) | 3 (50) |
| M. immunogenum | 1 | 0 | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 0 | 0 | 0 | 0 | 0 | 0 |
| M. llatzerense | 1 | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 0 | 0 | 0 | 0 | 0 | 0 |
| M. mucogenicum group | 16 | 4 (25) | 9 (56.3) | 12 (75) | 15 (93.8) | 15 (93.8) | 16 (100) | 0 | 0 | 1 (6.3) | 1 (6.3) | 1 (6.3) | 1 (6.3) |
| Total | 57 | 34 (59.6) | 43 (75.4) | 48 (84.2) | 52 (91.2) | 53 (93) | 54 (94.7) | 26 (45.6) | 28 (49.1) | 29 (50.9) | 29 (50.9) | 31 (54.4) | 31 (54.4) |
| Slowly growing mycobacteria | |||||||||||||
| M. avium complex | 48 | 4 (8.3) | 17 (35.4) | 24 (50) | 26 (54.2) | 28 (58.3) | 29 (60.4) | 6 (12.5) | 17 (35.4) | 27 (56.3) | 30 (62.5) | 30 (62.5) | 32 (66.7) |
| M. avium | 24 | 1 (4.2) | 5 (20.8) | 10 (41.7) | 10 (41.7) | 12 (50) | 13 (54.2) | 2 (8.3) | 6 (25) | 12 (50) | 15 (62.5) | 15 (62.5) | 17 (70.8) |
| M. colombiense | 1 | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) |
| M. intracellulare/M. chimaera | 22 | 3 (13.6) | 11 (50) | 13 (59.1) | 15 (68.2) | 15 (68.2) | 15 (68.2) | 4 (18.2) | 10 (45.5) | 14 (63.6) | 14 (63.6) | 14 (63.6) | 14 (63.6) |
| M. yongonense | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| M. gordonae | 14 | 0 | 0 | 1 (7.1) | 4 (28.6) | 8 (57.1) | 10 (71.4) | 0 | 0 | 0 | 0 | 0 | 0 |
| M. lentiflavum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 (100) | 1 (100) |
| M. paragordonae | 7 | 0 | 0 | 1 (14.3) | 2 (28.6) | 4 (57.1) | 4 (57.1) | 0 | 0 | 0 | 0 | 0 | 0 |
| M. paraffinicum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| M. simiae | 1 | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 0 | 1 (100) | 1 (100) | 1 (100) | 1 (100) | 1 (100) |
| Mycobacterium sp. | 4 | 0 | 0 | 1 (25) | 2 (50) | 3 (75) | 4 (100) | 0 | 0 | 0 | 0 | 0 | 1 (25) |
| Total | 76 | 4 (5.3) | 18 (23.7) | 28 (36.8) | 35 (46.1) | 44 (57.9) | 48 (63.2) | 6 (7.9) | 18 (23.7) | 28 (36.8) | 31 (40.8) | 32 (42.1) | 35 (46.1) |
| Grand total | 133 | 38 (28.6) | 61 (45.9) | 76 (57.1) | 87 (65.4) | 97 (72.9) | 102 (76.7) | 32 (24.1) | 46 (34.6) | 57 (42.9) | 60 (45.1) | 63 (47.4) | 66 (49.6) |
a
That is, the total number of nontuberculous mycobacteria isolated from a combination of all culture test methods (RGM30, RGM35, the MGIT system, and Middlebrook 7H11 agar).