TABLE 1.
Identification results and performance of detection for organisms targeted by the ePlex BCID panels compared to culture
| ePlex panel (no. of cartridges tested with valid results) and positive targets by ePlex | Isolate identification on culture (n) | Isolates not detected by ePlex (n) | Sensitivity (%) | Specificity (%) |
|---|---|---|---|---|
| BCID-GP (93) | ||||
| Staphylococcus, S. aureus | S. aureus (14) | 14/14 (100) | 79/79 (100) | |
| Staphylococcus, S. epidermidis | S. epidermidis (20) | S. epidermidis (1) | 20/21 (95) | 72/72 (100) |
| Staphylococcusb | S. haemolyticus (5) | 40/40b (100) | 53/53b (100) | |
| Streptococcus, S. pyogenes | S. pyogenes (1) | 1/1 (100) | 92/92 (100) | |
| Streptococcus, S. agalactiae | S. agalactiae (3) | 3/3 (100) | 90/90 (100) | |
| Streptococcus, S. pneumoniae | S. pneumoniae (9) | 9/9 (100) | 84/84 (100) | |
| Streptococcus, S. anginosus group | S. anginosus (2), S. intermedius (1) | 3/3 (100) | 90/90 (100) | |
| Streptococcusb | S. gallolyticus (4), S. dysgalactiae (3), S. mitis (3), S. lutetiensis (1) | 27/27b (100) | 66/66b (100) | |
| Enterococcus, E. faecalis | E. faecalis (17) | 17/17 (100) | 76/76 (100) | |
| Enterococcus, E. faecium | E. faecium (13) | 13/13 (100) | 80/80 (100) | |
| Enterococcusb | None | 30/30b (100) | 63/63b (100) | |
| Corynebacterium | C. mucifaciens (1) | 1/1 (100) | 92/92 (100) | |
| Listeria, L. monocytogenes | L. monocytogenesa (1) | 1/1 (100) | 92/92 (100) | |
| Lactobacillus | Not cultured (1) | NAc | 92/93 (99) | |
| Pan-Gram negative | E. coli (3), P. mirabilis (1), Bacteroides caccae (1) | E. coli (1) | 5/6 (83) | 87/87 (100) |
| BCID-GN (122) | ||||
| E. coli | E. coli (59) | 59/59 (100) | 63/63 (100) | |
| K. pneumoniae | K. pneumoniae (14) | 14/14 (100) | 108/108 (100) | |
| K. oxytoca | K. oxytoca (5) | 5/5 (100) | 117/117 (100) | |
| E. cloacae complex | E. cloacae (5), E. asburiae (1) | 6/6 (100) | 116/116 (100) | |
| Enterobacter (non-cloacae complex) | E. aerogenes (3) | 3/3 (100) | 119/119 (100) | |
| Citrobacter | C. koseri (1) | 1/1 (100) | 121/121 (100) | |
| Proteus, P. mirabilis | P. mirabilis (8) | 8/8 (100) | 114/114 (100) | |
| M. morganii | M. morganii (2) | 2/2 (100) | 120/120 (100) | |
| Serratia, S. marcescens | S. marcescens (3) | 3/3 (100) | 119/119 (100) | |
| Serratiab | S. liquefaciens (1) | 4/4 (100)b | 118/118 (100)b | |
| P. aeruginosa | P. aeruginosa (13) | P. aeruginosa (1) | 13/14 (93) | 108/108 (100) |
| S. maltophilia | S. maltophiliaa (2) | 2/2 (100) | 120/120 (100) | |
| B. fragilis | B. fragilisa (4) | 4/4 (100) | 118/118 (100) | |
| F. necrophorum | Not cultured (4) | NA | 118/122 (97) | |
| Pan-Gram positive | E. faecalis (3), E. faecium (1), E. avium (1), S. anginosus (1), S. gallolyticus (1), not cultured (2) | 7/7 (100) | 112/114 (98) | |
| BCID-FP (7) | ||||
| C. albicans | C. albicans (2) | 2/2 (100) | 5/5 (100) | |
| C. glabrata | C. glabrata (3) | 3/3 (100) | 4/4 (100) | |
| C. parapsilosis | C. parapsilosis (1) | 1/1 (100) | 6/6 (100) |
a
Including one isolate recovered at secondary subculture of frozen blood culture sample aliquots.
b
Performance calculated including all isolates belonging to the targeted genus.
c
NA, not applicable.