TABLE 3.
Diagnostic accuracy of metagenomic analysis versus culture of sonicate fluid for diagnosis of prosthetic joint infectiona
| Date set (no. of subjects) | Method | Sensitivity (% [95% CI]) | Specificity (% [95% CI]) | PPV (% [95% CI]) | NPV (% [95% CI]) |
|---|---|---|---|---|---|
| Derivation set (204) | Metagenomics | 69.9 (60.1, 78.6)b | 95.0 (88.3, 98.4) | 93.5 (84.9, 97.2) | 75.6 (69.7, 80.7) |
| Culture | 58.3 (48.1, 67.9)b | 100.0 (96.4, 100.0) | 100.0 (92.5, 100.0) | 70.1 (65.2, 74.7) | |
| Validation set (204) | Metagenomics | 67.3 (57.7,75.9)b | 89.4 (81.3, 94.8)b | 88.1 (80.2, 93.1) | 70.0 (63.9, 75.5) |
| Culture | 50.0 (40.3, 59.7)b | 100.0 (96.2, 100)b | 100.0 (91.7, 100.0) | 63.1 (58.6, 67.3) | |
| Overall (408) | Metagenomics | 68.5 (61.9, 74.7)b | 92.3 (87.6, 95.6)b | 90.7 (85.6, 94.1) | 72.9 (68.7, 76.7) |
| Culture | 54.0 (47.1, 60.8)b | 100.0 (98.1, 100.0)b | 100.0 (95.9, 100.0) | 66.6 (63.2, 69.7) |
a
CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value.
b
Statistically significant difference from the other test in the same data set (P < 0.05).