TABLE 3.
Total C. glabrata cell sterol composition in an isolate (Kw1421/16) wild type for AMB and 7 isolates with reduced susceptibility to AMB
| Type of sterol | % of each sterol as part of the total cell sterol composition of C. glabrata isolatea: |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCC 90030 | Kw280/06 | Kw1421/16 | Kw844/10 | Kw861/13 | Kw96/15 | Kw1856/15 | Kw2516/15 | Kw2813/15 | Kw3060/15 | Kw3357/15 | |
| Ergosta-5,8,22,24-tetraenol | 0.9 + 0.1 | 3.4 + 1.2 | 0.7 + 0.1 | 1.5 + 0.0 | 1.2 + 0.3 | ||||||
| Unknown sterol | 1.4 + 0.1 | 1.4 + 0.0 | 0.9 + 0.1 | 1.2 + 0.0 | 1.0 + 0.1 | 0.9 + 0.0 | |||||
| Ergosta-5,8,22-trienol | 0.9 + 0.0 | 1.0 + 0.0 | 0.4 + 0.3 | 25.8 + 0.3 | 31.6 + 1.1 | ||||||
| Ergosta-8,22-dienol | 7.0 + 0.7 | 8.3 + 0.2 | |||||||||
| Ergosta-5,8,24-trienol | 8.2 + 0.5 | 8.0 + 1.4 | |||||||||
| Ergosta-5,8-dienol | 6.0 + 0.4 | 1.3 + 0.1 | |||||||||
| Cholesta-5,8,24-trienol | 5.1 + 0.3 | 9.0 + 0.3 | 5.2 + 0.5 | 9.5 + 0.5 | 6.0 + 0.4 | 6.5 + 0.1 | |||||
| Zymosterol (cholesta-8,24-dienol) | 2.5 + 0.6 | 21.4 + 0.8 | 14.5 + 0.5 | 42.5 + 1.3 | 35.5 + 0.6 | 42.7 + 0.4 | 45.4 + 1.0 | ||||
| Cholesta-5,7,24-trienol | 1.1 + 0.0 | 1.6 + 1.3 | 56.0 + 0.4 | 40.9 + 0.5 | 34.0 + 0.2 | 36.9 + 0.7 | 35.2 + 0.3 | 36.3 + 1.3 | |||
| Ergosterol | 93.0 + 0.1 | 84.8 + 2.8 | 89.5 + 7.2 | ||||||||
| Cholesta-7,24-dienol | 1.3 + 0.5 | 1.0 + 0.1 | 1.9 + 0.3 | 0.8 + 0.0 | 1.4 + 0.1 | 1.0 + 0.0 | |||||
| Cholesta-5,7,22,24-tetraenol | 0.1 + 0.2 | 26.7 + 0.9 | 11.5 + 0.8 | 13.6 + 1.6 | 10.3 + 0.5 | 6.8 + 0.4 | |||||
| 14-Methyl fecosterol | 1.0 + 0.0 | ||||||||||
| Fecosterol [ergosta-8,24(28)-dienol] | 1.0 + 0.0 | 2.0 + 0.3 | 2.1 + 1.6 | 14.9 + 2.2 | 18.9 + 2.3 | ||||||
| Ergosta-8-enol | 30.9 + 1.3 | 29.5 + 0.9 | |||||||||
| Ergosta-5,7-dienol | 1.3 + 0.2 | 1.8 + 0.1 | 2.2 + 1.7 | 0.3 + 0.3 | 5.9 + 0.3 | 0.4 + 0.1 | 0.6 + 0.1 | ||||
| Episterol [ergosta-7,24(28)-dienol] | 0.2 + 0.0 | 0.7 + 0.1 | 1.2 + 0.9 | ||||||||
| Unknown sterol | 2.1 + 0.3 | 0.7 + 0.2 | |||||||||
| 14-Methyl ergosta-8,24(28)-dien-3,6-diol | |||||||||||
| Lanosterol | 1.3 + 0.1 | 3.2 + 0.3 | 2.1 + 1.6 | 2.5 + 0.1 | 11.6 + 1.0 | 0.6 + 0.1 | 3.3 + 0.3 | 1.3 + 0.2 | 2.4 + 0.1 | 1.2 + 0.3 | 2.1 + 0.3 |
| 4,4-Dimethyl cholesta-8,24-dienol | 0.2 + 0.1 | 0.6 + 0.4 | 0.2 + 0.2 | 1.1 + 0.1 | 1.9 + 0.2 | 0.8 + 0.2 | 0.4 + 0.1 | 0.7 + 0.0 | 0.3 + 0.0 | ||
a
C. glabrata strains ATCC 90030 and Kw280/06 were used as the reference strains for determining the total cell sterol composition. Sterol values (means for 3 replicates with standard deviation) are the percentage of the total sterol composition, and values of >5% of the total cell sterol composition are shown in bold. AMB, amphotericin B.