FIG 3.

Inhibition of host isoprenoid biosynthesis with atorvastatin results in growth kinetics that deviate from delayed death. Parasite growth was quantified by flow cytometry of T. gondii parasites manually released from host cells at each time point after treatment with atorvastatin and in the presence or absence of apicoplast drugs. Growth is normalized to that of parasites treated with the same apicoplast inhibitor but in the absence of atorvastatin at each time point. Data are representative of results from two biological replicates. Error bars represent the SEM. Because host cell lysis occurs between 36 and 48 h, parasites analyzed at these time points may reflect growth in the second lytic cycle.