FIG 3.

Sofosbuvir inhibits CHIKV-induced cell death and replication in iPSC-derived human astrocytes. (A and B) Astrocytes were infected at the indicated MOIs and treated with sofosbuvir at 10 μM. After 3 days, cells were labeled for activated caspase-3/7 and propidium iodide (A) and titers of virus in the supernatant were determined in Vero cells (B). (C and D) Astrocytes were infected at an MOI of 1.0 and treated with sofosbuvir or ribavirin at the indicated concentrations. After 3 days, cells death (C) and virus titers in Vero cells (D) were determined. The data represent means ± SEM from five independent experiments performed with five technical replicates. *, P < 0.05 for comparisons between infected, untreated (gray bars) and treated (black bars) groups (A and B) and for comparisons between groups infected/treated with sofosbuvir (open squares) and ribavirin (black squares) (C and D).