FIG 6.
Sofosbuvir, at concentrations of 40 and 80 mg/kg/day, increased survival and inhibited weight loss of CHIKV-infected mice. Three-day-old Swiss mice were infected with CHIKV (2 × 102 PFU) and treated with sofosbuvir (SF) either 1 day before (pretreatment) or 2 days after infection (late treatment). Survival (A and C) and weight variation (B and D) were assessed during the course of treatment. (A and B) Experiments of both pretreatment and late treatment with sofosbuvir at 20 mg/kg/day. (C and D) Pretreatment with the indicated concentrations of sofosbuvir. Survival was statistically assessed by log-rank (Mentel-Cox) test. Differences in weight are displayed as the means ± SEM, and two-way ANOVA for each day was used to assess significance. Independent experiments were performed with at least 10 mice/group (n = 30). *, P < 0.01.