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. 2018 Nov 28;39:520–530. doi: 10.1016/j.ebiom.2018.11.038

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Expression of ERα isoforms and GJA1 in pregnant human myometrium. Representative western blot (A) and densitometric analysis (B) demonstrate ERΔ7 protein in nuclear (Nuc) extracts significantly declined in term laboring (TL) and term non-laboring (TNL) myometrium compared to myometrium from preterm non-laboring (PTNL) women. (C) ERΔ7 mRNA levels are also down-regulated in the TNL samples as compared to PTNL. Representative western blot (D) and densitometric analysis demonstrated no significant change in ERα66 levels in cytoplasmic (Cyto) (E) and nuclear (F) fractions of TL and TNL myometrium as compared to PTNL. Representative western blot (G) and densitometric analysis (H) demonstrated cytoplasmic GJA1 expression levels were significantly upregulated in the TL and TNL as compared to PTNL myometrium. An increasing trend in GJA1 mRNA levels (I) was also observed towards term. GAPDH and NCOA3 are cytoplasmic and nuclear loading controls. Gene expression was normalized to Rplp0. *p < 0.05 using one-way ANOVA, followed by the Newman-Keuls multiple-comparison test; N = 20 per group. Data shown are mean ± SEM.