Fig. 5.

hnRNPG expression declines in myometrium from women at term. Representative western blot (A) and densitometric analysis (B) demonstrates a significant decline in hnRNPG protein levels in nuclear extracts of term laboring (TL) and term non-laboring (TNL) myometrium compared to myometrium from preterm non-laboring (PTNL) women. Q-PCR analysis (C) also demonstrates a significant decline in hnRNPG TNL myometrium compared to myometrium from PTNL women. Representative western blot (D) and densitometric analysis demonstrating a significant decline in hnRNPG (E) and ERΔ7 (F) protein levels upon estradiol (E2) treatment in the hTERT-HM cell. hTERT-HM cells were treated with vehicle, progesterone (100 nM; P4) or E2 (100 nM) for 24 h. Nuclear extracts were prepared and analyzed for hnRNPG and ERΔ7 proteins. NCOA3 is used as nuclear loading control. Gene expression was normalized to Rplp0. *p < 0.05 using one-way ANOVA, followed by the Newman-Keuls multiple-comparison test; each experiment was performed in triplicate with n = 4 per group. Data shown are mean ± SEM.