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. 2018 Dec 5;39:59–68. doi: 10.1016/j.ebiom.2018.11.056

Fig. 1.

Fig. 1

HO-1 repressed replicative cardiomyocytes senescence. Primary cardiomyocytes were cultured for 3 or 10 days and treated with or without hemin (10 μM) every other day. (a) Hemin induced production of HO-1 taken at the 10-day time point was shown by western blot. n = 3. **P < .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis. (b) The effect of HO-1 on expression levels of senescence markers containing LaminB, p53 and p16 were analyzed by western blot. n = 3. *P < .01, **P < .05. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (c) and (d) SA-β-gal activity was determined using Senescence β-Galactosidase Staining Kit. Scale bars represent 100 μm. n = 5. *P < .01, **P < .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis.