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. 2018 Dec 5;39:59–68. doi: 10.1016/j.ebiom.2018.11.056

Fig. 3.

Fig. 3

Up-regulation of HO-1 attenuated heart aging in vivo. The systemic HO-1 transgenic overexpression aged mice were 16 months. (a) The protein levels of LaminB, p53 and p16 were analyzed by western blot. n = 3. **P < .05. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (b) Immunofluorescence staining using anti-p16 and anti-α-actinin antibodies showed the level change of p16. The tissue section thickness is 6 μm. Scale bars represent 100 μm. (c) Immunohistochemistry staining using anti-p16 antibody showed the level change of p16. The tissue section thickness is 6 μm. Scale bars represent 100 μm. (d) Relative levels of SASP containing IL-1, IL-3 and TNF-α were analyzed by qRT-PCR. n = 3 to 4. *P < .01. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis.