Fig. 4.

HO-1 repressed cardiomyocytes senescence under the treatment of H₂O₂ in vitro. Primary cardiomyocytes were treated with H₂O₂ (50 μM) for 3 h and hemin (10 μM) for 24 h. (a) The expression levels of LaminB, p53 and p16 analyzed by western blot. n = 3. *P < .01, **P < .05. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (b) and (c) SA-β-gal activity analyzed by Senescence β-Galactosidase Staining Kit. Scale bars represent 100 μm n = 4. **P < .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis.