Fig. 5.

Overproduction of HO-1 induced by hemin improves heart function of mice with MI injury. Mice were 6–8 weeks. The model of MI was established by ligating left anterior descending coronary artery for a week. Mice in MI + Hemin group were i.p. injected with 20 mg/kg (once every other day) of hemin. (a) Representative short-axis M-mode images from Sham, MI and MI + Hemin group. (b) Ejection Fraction (EF) and Fractional Shortening (FS) of Sham, MI and MI + Hemin group were detected by Echocardiographic assessment. n = 4. **P < .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis. (c) Relative mRNA levels of Col 1α1and Col 3α1 analyzed by qRT-PCR. n = 4. **P < .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis. (d) Masson staining showed the deposition of ECM. Scale bars represent 50 μm. n = 3.