Figure 2.

Effect of LPS induced neuroinflammation in the expression profiles of inflammatory markers and GILZ: groups of adult C57BL/6 mice were subjected to LPS induced neuroinflammation as described in the “Materials and Methods” section. Equal quantity of cDNA isolated from brain tissues were amplified for IL-1β, CD14, toll like receptor-4 (TLR-4), IL-12, CD4 and β-actin mRNA by quantitative real time polymerase chain reaction (PCR). (A) Gel electrophoresis of the PCR products β-actin IL-1β, CD14, TLR-4, GILZ, IL-12 and CD4. Panel (B) shows indicated inflammatory cytokines in the circulation as determined by enzyme linked immunosorbent assay (ELISA). (C–H) Relative mRNA quantitation of the indicated product with respect to that of housekeeping gene β-actin is shown. Data are average ± SD. * and ^@p < 0.05 with respect to the vehicle treated group or naïve group respectively. Panel (I,J) shows data from regression analysis for GILZ and TLR4 transcripts in mice administered saline (I) or LPS (J).