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. 2019 Jan 25;12:6. doi: 10.3389/fnmol.2019.00006

FIGURE 3.

FIGURE 3

A chromaffin body located between the suprarenal and mesenteric ganglion is Schwann-cell-precursor-derived. (A) Immunofluorescence on cryosections against Plp1YFP, CART and TH on an E15.5 embryo injected with tamoxifen (TAM) at E11.5 showing the sympathetic ganglion (SG), suprarenal ganglion (SRG) and mesenteric ganglion (MG). (B) Quantification of recombination in chromaffin and sympathetic population in embryos TAM-injected on E11.5 and analyzed at E15.5 (SG 2.72 ± 0.65%, SRG 2.35 ± 1.04%, MG 1.96 ± 0.81%, TCB 45.09 ± 4.90%, in all cases N = 4). Data are presented as mean ± SEM. In TCB vs. SG P = 0.0004, TCB vs. SRG P = 0.0003 and TCB vs. MG P = 0.0005. (C) Immunofluorescence on cryosections against Plp1YFP, CART and TH on an E15.5 embryo injected with TAM at E11.5 at the area of transition from the SRG to the MG, showing the “transitional chromaffin body” (TCB). Note the differential CART and TH signal between the SRG and MG, as well as the Plp1YFP+/THhigh cells in the TCB. Note the high percentage of Plp1YFP+/TH+ cells in the TCB and the absence of Plp1YFP+/TH+ cells in the SG, SRG and MG. Scale bar in (A,C) = 50 μm. SG, sympathetic ganglion; SRG, suprarenal ganglion; MG, mesenteric ganglion; TCB, transitional chromaffin body; ∗∗∗P ≤ 0.001.