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Number of potential off-target sites for mutations in A and B genomes of plantain for the three CRISPR targets in eBSOLV integrated in Gonja Manjaya (AAB)
| gRNA sequence from eBSOLV + PAM | Number of potential off-targets | |
|---|---|---|
| A genome | B genome | |
| TTGAGCAAAGAAGACGTTGAGG | 11 | 9 |
| GCTGGAACAACTGGTGACTGGG | 13 | 6 |
| ATGGAGTTCATATGATCATAGG | 34 | 25 |
The PAM sequences are shown in italics
