Fig. 5.

Novel Acidobacteria lineages. a Distribution of Acidobacteria classes in the biofilms and seawater. Based on 16S miTags, several rarely studied or unclassified groups are enriched in biofilms. b Phylogenetic analysis of Acidobacteria 16S rRNA gene sequences (over 1000 bp) extracted from assembled biofilm metagenomes. 16S rRNA gene sequences that are available for known Acidobacteria classes were downloaded from the NCBI database and used as references and Rokubacteria was used to root the tree. Black dots indicate bootstrap values above 60. These analyses provide further support for novel microbial lineages within ocean biofilms