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Validation of neuronal-specific genes that were induced during late stages of RML-scrapie infected mice. Fold change of 6 genes were calculated between RML infected and mock-infected (A) CA1 hippocampal and (B) cerebellar granular layer microdissected regions. GAPDH served as the normalization control for all validations. Data is represented as mean ± SEM (n = 4). A one-tailed unpaired t-test was used to calculate significance where *p < 0.05; **p < 0.01; ***p < 0.001.
