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. 2018 Mar 5;16(2):165–177. doi: 10.1038/cmi.2017.167

Figure 4.

Figure 4

NIK regulates the expression of CXCL13 in LECs. (a) Flow cytometry analysis of the frequency of LECs (CD31+gp38+), BECs (CD31+gp38−) and FRCs (CD31-gp38+) in the LNs of WT and NIKLEC-KO (KO) mice. Plots were gated on CD45.2− stromal cells. Data are presented as representative plots (left) and summary graphs (right). (b) Flow cytometry analysis of the expression of Lyve1 (represented by GFP expression) by LECs and BECs in the LNs of WT and NIKLEC-KO (KO) mice. (c) Flow cytometry analysis of the expression of ICAM-1 by LECs, BECs and FRCs in the LNs of WT and NIKLEC-KO (KO) mice. Data are presented as representative plots (left) and summary graphs for the MFI of ICAM-1 (right). (d) qRT-PCR analysis was performed using total RNA from LECs and BECs that were sorted from LNs of WT and NIKLEC-KO (KO) mice by flow cytometry. Data are presented as summary graphs for the expression of indicated genes normalized to the expression of the housekeeping gene Actb. Data are representative of at least three independent experiments and are presented as means±s.e.m. *P<0.05; **P<0.01; ***P<0.001. BEC, blood endothelial cell; FRC, fibroblast reticular cell; ICAM-1, intercellular adhesion molecule 1; LN, lymph node; KO, knockout; LEC, lymphatic endothelial cell; Lyve1, lymphatic endothelial hyaluronan receptor 1; MFI, mean fluorescence intensity; NIK, nuclear factor-κB-inducing kinase; NS, not significant, qRT-PCR, real-time quantitative PCR; WT, wild type.