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. 2019 Jan 31;9:1092. doi: 10.1038/s41598-018-37765-3

Figure 2.

Figure 2

Muscle-specific knockout of miR-23–27–24 clusters. (A) Representative images of the genotyping of the floxed and WT alleles of miR-23–27–24 clusters and the Ckmm-cre allele from mouse genomic DNA. (B) mRNA expression of Cre in liver, lung, plantaris (PL) and soleus (sol) of the WT and dKO mice. (C) PCR with genomic DNA to detect Cre-induced recombination in skeletal muscle. Other cells include myoblasts and fibroblasts, etc., M. fiber: isolated myofibers, Whole muscle: whole EDL muscle, Flox: floxed allele. KO: knockout allele. (D–H) Expression of mature miRNAs generated from miR-23–27–24 clusters; miR-23a, miR-23b, miR-24, miR-27a, and miR-27b in plantaris muscle (D) soleus muscle (E) myofibers (F) other cells in muscle tissue (G) and liver (H) of WT and dKO mice. Data are means ± SEM (n = 5–6). ##P < 0.01 compared with WT.